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验证数据展示

  • WB analysis of human testis using 83584-3-RR

    WB analysis of human testis using 83584-3-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 83584-3-RR

    FC experiment of PC-3 using 83584-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83584-3-RR

    Affinity and Kinetic Characterization of 83584-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

GLIPR1L1 Recombinant antibody

GLIPR1L1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83584-3-RR

产品说明书

CloneNo. 240594D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83584-3-RR targets GLIPR1L1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GLIPR1L1 fusion protein Ag14852 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GLI pathogenesis-related 1 like 1
别名 UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972
计算分子量 242 aa, 27 kDa
观测分子量25 kDa
GenBank蛋白编号BC014603
基因名称 GLIPR1L1
Gene ID (NCBI) 256710
RRIDAB_3671198
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6UWM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLIPR1L1 antibody 83584-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols