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GLIPR1L1 Recombinant antibody

GLIPR1L1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240594D10

Cat no : 83584-3-RR

Print datasheet

Synonyms

UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972

验证数据展示

  • WB analysis of human testis using 83584-3-RR

    WB analysis of human testis using 83584-3-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 83584-3-RR

    FC experiment of PC-3 using 83584-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83584-3-RR

    Affinity and Kinetic Characterization of 83584-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83584-3-RR targets GLIPR1L1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen GLIPR1L1 fusion protein Ag14852 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GLI pathogenesis-related 1 like 1
Synonyms UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972
Calculated Molecular Weight 242 aa, 27 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC014603
Gene Symbol GLIPR1L1
Gene ID (NCBI) 256710
RRIDAB_3671198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6UWM5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLIPR1L1 antibody 83584-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols