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GLIS1 Polyclonal antibody

GLIS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23138-1-AP

Print datasheet

Synonyms

GLI similar 1, GLIS family zinc finger 1, GLIS1, Zinc finger protein GLIS1

验证数据展示

  • IHC staining of mouse ovary using 23138-1-AP

    IHC staining of mouse ovary using 23138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 23138-1-AP

    WB analysis of Jurkat using 23138-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23138-1-AP is GLIS1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GLIS1 fusion protein Ag19214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GLIS family zinc finger 1
Synonyms GLI similar 1, GLIS family zinc finger 1, GLIS1, Zinc finger protein GLIS1
Calculated Molecular Weight 620 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC101799
Gene Symbol GLIS1
Gene ID (NCBI) 148979
RRIDAB_2879216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8NBF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLIS1 antibody 23138-1-APDownload protocol
IHC protocol for GLIS1 antibody 23138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Reprod Sci

Effect of GLIS1 on the Lymph Node Metastasis of Cervical Squamous Carcinoma Based on Transcriptome Analysis

Authors - Jiazhen Huang
View Article