验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, HeLa cells, rat liver tissue, HepG2 cells, mouse liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GLO1 Ready-To-Use IHC Kit. GLO1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15140-1-AP targets GLO1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GLO1 fusion protein Ag7314 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glyoxalase I |
| 别名 | Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, glyoxalase I, Ketone aldehyde mutase, Lactoylglutathione lyase, Methylglyoxalase |
| 计算分子量 | 21 kDa |
| 观测分子量 | 21-25 kDa |
| GenBank蛋白编号 | BC001741 |
| 基因名称 | GLO1 |
| Gene ID (NCBI) | 2739 |
| RRID | AB_2109890 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q04760 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer. The molecular mass of the dimer was 46 kDa determined by gel permeation chromatography.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLO1 antibody 15140-1-AP | Download protocol |
| IHC protocol for GLO1 antibody 15140-1-AP | Download protocol |
| IP protocol for GLO1 antibody 15140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Brain C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis. | ||
Front Pharmacol Protective effect of heat-processed Gynostemma pentaphyllum on high fat diet-induced glucose metabolic disorders mice | ||
Phytomedicine Protective effects of Huang-Lian-Jie-Du Decoction on diabetic nephropathy through regulating AGEs/RAGE/Akt/Nrf2 pathway and metabolic profiling in db/db mice. | ||
Oncotarget Glyoxalase 1 gene is highly expressed in basal-like human breast cancers and contributes to survival of ALDH1-positive breast cancer stem cells.
| ||
Life Sci Alliance Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications. | ||
Pharmacol Res Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy. |