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GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B7A8

Cat no : 68242-1-Ig

Print datasheet

Synonyms

GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5

验证数据展示

  • WB analysis using 68242-1-Ig

    WB analysis using 68242-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68242-1-Ig

    WB analysis of LNCaP using 68242-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68242-1-Ig

    WB analysis using 68242-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 68242-1-Ig

    FC experiment of HeLa using 68242-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug GLO1 Monoclonal antibody (68242-1-Ig, Clone:1B7A8) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 68242-1-Ig

    IF Staining of HeLa using 68242-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GLO1 antibody (68242-1-Ig, Clone: 1B7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GLO1 Ready-To-Use IHC Kit. GLO1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68242-1-Ig targets GLO1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GLO1 fusion protein Ag7314 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glyoxalase I
Synonyms GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21-25 kDa
GenBank Accession NumberBC001741
Gene Symbol GLO1
Gene ID (NCBI) 2739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.

实验方案

Product Specific Protocols
WB protocol for GLO1 antibody 68242-1-IgDownload protocol
IF protocol for GLO1 antibody 68242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols