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GLOD4 Polyclonal antibody

GLOD4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16788-1-AP

Print datasheet

Synonyms

C17orf25, CGI 150, GLOD4, glyoxalase domain containing 4, HC71, My027

验证数据展示

  • WB analysis using 16788-1-AP

    WB analysis using 16788-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16788-1-AP (GLOD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 16788-1-AP

    IHC staining of mouse ovary using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16788-1-AP (GLOD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 16788-1-AP

    IHC staining of human heart using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16788-1-AP

    IHC staining of human heart using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16788-1-AP (GLOD4 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of mouse ovary using 16788-1-AP

    IHC staining of mouse ovary using 16788-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16788-1-AP (GLOD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse kidney tissue, human placenta tissue, rat brain tissue
Positive IHC detected inhuman heart tissue, mouse ovary tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16788-1-AP targets GLOD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymonkey
Immunogen GLOD4 fusion protein Ag10222 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glyoxalase domain containing 4
Synonyms C17orf25, CGI 150, GLOD4, glyoxalase domain containing 4, HC71, My027
Calculated Molecular Weight 289 aa, 33 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC008605
Gene Symbol GLOD4
Gene ID (NCBI) 51031
RRIDAB_2247517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC38
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLOD4 antibody 16788-1-APDownload protocol
IHC protocol for GLOD4 antibody 16788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyIHC

Mol Biosyst

Unravelling the proteome of adult rhesus monkey ovaries.

Authors - Hui He
View Article