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验证数据展示

  • IHC staining of human ovary using 55292-1-AP

    IHC staining of human ovary using 55292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 55292-1-AP

    IHC staining of human ovary using 55292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of mouse ovary using 55292-1-AP

    WB analysis of mouse ovary using 55292-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55292-1-AP (ZGLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZGLP1 Polyclonal antibody

ZGLP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55292-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

55292-1-AP targets ZGLP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GATA like protein-1
别名 GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号NM_001103167
基因名称 GLP-1
Gene ID (NCBI) 100125288
RRIDAB_2881301
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0C6A0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZGLP1, also known as GLP-1, is a nuclear protein containing two zinc fingers, one of which is highly homologous to the conserved GATA zinc finger DNA binding motif. It is a transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development.

实验方案

Product Specific Protocols
WB protocol for ZGLP1 antibody 55292-1-APDownload protocol
IHC protocol for ZGLP1 antibody 55292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Chin J Integr Med

Huanglian-Renshen-Decoction Maintains Islet β-Cell Identity in T2DM Mice through Regulating GLP-1 and GLP-1R in Both Islet and Intestine

Authors - Wen-Bin Wu
View Article
mouseIHC

Front Pharmacol

Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell

Authors - Wenbin Wu
View Article