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ZGLP1 Polyclonal antibody

ZGLP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55292-1-AP

Print datasheet

Synonyms

GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1

验证数据展示

  • IHC staining of human ovary using 55292-1-AP

    IHC staining of human ovary using 55292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 55292-1-AP

    IHC staining of human ovary using 55292-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 55292-1-AP (GLP1 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of mouse ovary using 55292-1-AP

    WB analysis of mouse ovary using 55292-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 55292-1-AP (ZGLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse ovary tissue
Positive IHC detected inhuman ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

55292-1-AP targets ZGLP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GATA like protein-1
Synonyms GATA like protein 1, GLP 1, GLP1, GLP-1, ZGLP1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberNM_001103167
Gene Symbol GLP-1
Gene ID (NCBI) 100125288
RRIDAB_2881301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0C6A0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZGLP1, also known as GLP-1, is a nuclear protein containing two zinc fingers, one of which is highly homologous to the conserved GATA zinc finger DNA binding motif. It is a transcriptional repressor that plays a central role in somatic cells of the gonad and is required for germ cell development.

实验方案

Product Specific Protocols
WB protocol for ZGLP1 antibody 55292-1-APDownload protocol
IHC protocol for ZGLP1 antibody 55292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Front Pharmacol

Berberine enhances the function of db/db mice islet β cell through GLP-1/GLP-1R/PKA signaling pathway in intestinal L cell and islet α cell

Authors - Wenbin Wu
View Article