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验证数据展示

  • WB analysis of A549 using 12855-1-AP

    WB analysis of A549 using 12855-1-AP

    WB result of KGA/GAC antibody (12855-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KGA/GAC transfected A549 cells.

  • IP experiment of mouse brain using 12855-1-AP

    IP experiment of mouse brain using 12855-1-AP

    IP result of anti-KGA/GAC (IP:12855-1-AP, 4ug; Detection:12855-1-AP 1:5000) with mouse brain tissue lysate 2400 ug.

  • IHC staining of human gliomas using 12855-1-AP

    IHC staining of human gliomas using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • IHC staining of human gliomas using 12855-1-AP

    IHC staining of human gliomas using 12855-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12855-1-AP (KGA/GAC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KGA/GAC antibody included
  • WB analysis of HEK-293 using 12855-1-AP

    WB analysis of HEK-293 using 12855-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.

  • WB analysis using 12855-1-AP

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 12855-1-AP

    IF Staining of HEK-293 using 12855-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12855-1-AP (KGA/GAC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12855-1-AP

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12855-1-AP

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • WB analysis using 12855-1-AP

    WB analysis using 12855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12855-1-AP (KGA/GAC antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 12855-1-AP

    FC experiment of HeLa using 12855-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human KGA/GAC (12855-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

KGA/GAC Polyclonal antibody

KGA/GAC Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 12855-1-AP

产品说明书

引用文献(104)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

GLS, KGA, GLS1, GAM, GAC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, A549 cells, COLO 320 cells, HEK-293 cells, mouse kidney tissue, rat brain tissue, rat liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, human kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, L02 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KGA/GAC Ready-To-Use IHC Kit. KGA/GAC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12855-1-AP targets KGA/GAC in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, cat
免疫原 KGA/GAC fusion protein Ag3505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaminase
别名 GLS, KGA, GLS1, GAM, GAC
计算分子量 669 aa, 73 kDa
观测分子量58 kDa, 65 kDa
GenBank蛋白编号BC038507
基因名称 GLS
Gene ID (NCBI) 2744
RRIDAB_2110381
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94925
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.

实验方案

Product Specific Protocols
WB protocol for KGA/GAC antibody 12855-1-APDownload protocol
IHC protocol for KGA/GAC antibody 12855-1-APDownload protocol
IF protocol for KGA/GAC antibody 12855-1-APDownload protocol
IP protocol for KGA/GAC antibody 12855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IP

Cell Res

AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine

Authors - Mengqi Li
View Article
humanWB

Nature

CFIm25 links alternative polyadenylation to glioblastoma tumour suppression.

Authors - Chioniso P Masamha
View Article
mouseIF

Cell

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Authors - Haibo Zhou
View Article
mouseWB

Cell

Systemic elevation of PTEN induces a tumor-suppressive metabolic state.

Authors - Garcia-Cao Isabel I
View Article
WB

Cell Metab

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Authors - Qiuying Chen
View Article
mouseWB

Nat Metab

Quantitative analysis of metabolic fluxes in brown fat and skeletal muscle during thermogenesis

Authors - Grace Park
View Article