验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, A549 cells, Hela cells, MCF-7 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29519-1-AP targets GLS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GLS fusion protein Ag31167 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutaminase |
| 别名 | KGA, GLSK, GLS1, GAM, GAC |
| 计算分子量 | 73 kDa |
| 观测分子量 | 58 kDa, 65 kDa |
| GenBank蛋白编号 | NM_014905 |
| 基因名称 | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_2918320 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O94925 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLS antibody 29519-1-AP | Download protocol |
| IF protocol for GLS antibody 29519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer | ||
Front Pharmacol A novel method for generating glutamatergic SH-SY5Y neuron-like cells utilizing B-27 supplement | ||
Gen Comp Endocrinol Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats | ||
Mol Carcinog Glutaminase potentiates the glycolysis in esophageal squamous cell carcinoma by interacting with PDK1 | ||
Sci Rep Identification of subclusters and prognostic genes based on GLS-associated molecular signature in ulcerative colitis |