验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, A549 cells, Hela cells, MCF-7 cells, SH-SY5Y cells |
| Positive IF/ICC detected in | L02 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29519-1-AP targets GLS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | GLS fusion protein Ag31167 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | glutaminase |
| Synonyms | KGA, GLSK, GLS1, GAM, GAC |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 58 kDa, 65 kDa |
| GenBank Accession Number | NM_014905 |
| Gene Symbol | GLS |
| Gene ID (NCBI) | 2744 |
| RRID | AB_2918320 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94925 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLS antibody 29519-1-AP | Download protocol |
| IF protocol for GLS antibody 29519-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer | ||
Front Pharmacol A novel method for generating glutamatergic SH-SY5Y neuron-like cells utilizing B-27 supplement | ||
Gen Comp Endocrinol Testosterone regulates thymic remodeling by altering metabolic reprogramming in male rats | ||
Mol Carcinog Glutaminase potentiates the glycolysis in esophageal squamous cell carcinoma by interacting with PDK1 | ||
Sci Rep Identification of subclusters and prognostic genes based on GLS-associated molecular signature in ulcerative colitis |