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GLS2 Polyclonal antibody

GLS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20171-1-AP

Print datasheet

Synonyms

GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA

验证数据展示

  • WB analysis using 20171-1-AP

    WB analysis using 20171-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20171-1-AP (GLS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 20171-1-AP

    IHC staining of human liver using 20171-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20171-1-AP targets GLS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen GLS2 fusion protein Ag14003 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutaminase 2 (liver, mitochondrial)
Synonyms GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight66-70 kDa
GenBank Accession NumberBC059370
Gene Symbol GLS2
Gene ID (NCBI) 27165
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UI32
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS2, also named as GA and GLS, belongs to the glutaminase family. It is Glutaminase liver isoform . GLS2 plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. GLS2 may play a role in preventing tumor proliferation. This antibody is specific to GLS2.

实验方案

Product Specific Protocols
WB protocol for GLS2 antibody 20171-1-APDownload protocol
IHC protocol for GLS2 antibody 20171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols