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验证数据展示

  • WB analysis using 20171-1-AP

    WB analysis using 20171-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20171-1-AP (GLS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 20171-1-AP

    IHC staining of human liver using 20171-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GLS2 Polyclonal antibody

GLS2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20171-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20171-1-AP targets GLS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GLS2 fusion protein Ag14003 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaminase 2 (liver, mitochondrial)
别名 GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA
计算分子量 602 aa, 66 kDa
观测分子量66-70 kDa
GenBank蛋白编号BC059370
基因名称 GLS2
Gene ID (NCBI) 27165
RRIDAB_3085600
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UI32
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS2, also named as GA and GLS, belongs to the glutaminase family. It is Glutaminase liver isoform . GLS2 plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. GLS2 may play a role in preventing tumor proliferation. This antibody is specific to GLS2.

实验方案

Product Specific Protocols
WB protocol for GLS2 antibody 20171-1-APDownload protocol
IHC protocol for GLS2 antibody 20171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols