验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16768-1-AP targets GLT25D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GLT25D1 fusion protein Ag10346 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycosyltransferase 25 domain containing 1 |
| 别名 | PSEC0241, EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 1, COLGALT1, ColGalT 1 |
| 计算分子量 | 231 aa, 27 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC020492 |
| 基因名称 | GLT25D1 |
| Gene ID (NCBI) | 79709 |
| RRID | AB_2878313 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NBJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IHC protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IF protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| IP protocol for GLT25D1 antibody 16768-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ann Neurol Biallelic COLGALT1 variants are associated with cerebral small vessel disease. | ||
J Mol Cell Cardiol Ascorbate starvation alters endoplasmic reticulum-resident enzymes in cardiac fibroblasts, priming them for increased procollagen secretion. | ||
PLoS Genet Cyclophilin B control of lysine post-translational modifications of skin type I collagen. | ||
Sci Rep Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes | ||
Biochemistry Role of Glycosyltransferase 25 domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.
| ||
Mol Med Rep Downregulation of Glt25d1 aggravates carbon tetrachloride‑induced acute hepatic injury through activation of the TGF‑β1/Smad2 signaling pathway.
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