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GLT25D2 Polyclonal antibody

GLT25D2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25993-1-AP

Print datasheet

Synonyms

EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 2, COLGALT2, ColGalT 2, C1orf17

验证数据展示

  • WB analysis of SH-SY5Y using 25993-1-AP

    WB analysis of SH-SY5Y using 25993-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25993-1-AP (GLT25D2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25993-1-AP (GLT25D2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 25993-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25993-1-AP (GLT25D2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSH-SY5Y cells, Neuro-2a cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25993-1-AP targets GLT25D2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen GLT25D2 fusion protein Ag23360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycosyltransferase 25 domain containing 2
Synonyms EC:2.4.1.50, Collagen beta(1-O)galactosyltransferase 2, COLGALT2, ColGalT 2, C1orf17
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC035672
Gene Symbol GLT25D2
Gene ID (NCBI) 23127
RRIDAB_2880324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLT25D2 antibody 25993-1-APDownload protocol
IHC protocol for GLT25D2 antibody 25993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Dis Model Mech

Loss of function of Colgalt1 disrupts collagen post-translational modification and causes musculoskeletal defects.

Authors - Krista A Geister
View Article