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验证数据展示

  • WB analysis using 30349-1-AP

    WB analysis using 30349-1-AP

    Untreated, and PMA treated treated NK-92 cells were subjected to SDS PAGE followed by western blot with 30349-1-AP (GM-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GM-CSF Polyclonal antibody

GM-CSF Polyclonal Antibody for WB, ELISA
Cat No. 30349-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNK-92 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30349-1-AP targets GM-CSF in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim
Observed Molecular Weight15kDa,18 kDa ,22 kDa
Gene Symbol GM-CSF
Gene ID (NCBI) 1437
RRIDAB_3669715
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

实验方案

Product Specific Protocols
WB protocol for GM-CSF antibody 30349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols