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验证数据展示

  • WB analysis using 30349-1-AP

    WB analysis using 30349-1-AP

    Untreated, and PMA treated treated NK-92 cells were subjected to SDS PAGE followed by western blot with 30349-1-AP (GM-CSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GM-CSF Polyclonal antibody

GM-CSF Polyclonal Antibody for WB, ELISA
Cat No. 30349-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNK-92 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30349-1-AP targets GM-CSF in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 colony stimulating factor 2 (granulocyte-macrophage)
别名 Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim
观测分子量15kDa,18 kDa ,22 kDa
基因名称 GM-CSF
Gene ID (NCBI) 1437
RRIDAB_3669715
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts. GM-CSF is a hematopoietic growth factor that stimulates the development of early erythroid megakaryocytic and eosinophilic progenitor cells (PMID 2990035). GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes (PMID 3021817, 2984574, 6390681).

实验方案

Product Specific Protocols
WB protocol for GM-CSF antibody 30349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols