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GM-CSF Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 15.6-1000 pg/mL

Cat No : MP00521-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00521-3, Human GM-CSF Recombinant Matched Antibody Pair - PBS only. 82761-11-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0189. 82761-8-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

    Sandwich ELISA standard curve of MP00521-3

    Sandwich ELISA standard curve of MP00521-3, Human GM-CSF Recombinant Matched Antibody Pair - PBS only. 82761-11-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0189. 82761-8-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean GM-CSF concentration was determined to be 55.0 pg/mL in unstimulated PBMC supernatant, 1821.6 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP00521-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean GM-CSF concentration was determined to be 55.0 pg/mL in unstimulated PBMC supernatant, 1821.6 pg/mL in PHA stimulated PBMC supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00521-3 targets GM-CSF in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 15.6-1000 pg/mL (Sandwich ELISA);

Capture antibody: GM-CSF Recombinant antibody, PBS Only (Capture) 82761-11-PBS (240183C1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: GM-CSF Recombinant antibody, PBS Only (Detector) 82761-8-PBS (240183A3). 100 μg. Concentration 1 mgl/ml.

Alternative GM-CSF matched antibody pairs: MP00306-1, MP00306-2, MP00306-3, MP00521-1, MP00521-2, MP50067-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 82761-11-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2
Gene symbol GM-CSF
UniprotP04141
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 82761-8-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Synonyms CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2
Gene symbol GM-CSF
UniprotP04141
Gene ID (NCBI) 1437
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C