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GOLGA2/GM130 Monoclonal antibody

GOLGA2/GM130 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66662-1-Ig

产品说明书

CloneNo. 2A4F11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat and More (1)

应用

WB, IF/ICC, ELISA and More (1)

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 2A4F11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, HSC-T6 cells, K-562 cells, PC-12 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLGA2/GM130 Ready-To-Use IHC Kit. GOLGA2/GM130 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66662-1-Ig targets GOLGA2/GM130 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, rat
文献引用反应性human, mouse, rat
免疫原 GOLGA2/GM130 fusion protein Ag1848 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 2
别名 GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 2A4F11
计算分子量 111 kDa
观测分子量 130 kDa
GenBank蛋白编号BC014188
基因名称 GM130
Gene ID (NCBI) 2801
RRIDAB_2882017
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ08379
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

实验方案

Product Specific Protocols
WB protocol for GOLGA2/GM130 antibody 66662-1-IgDownload protocol
IF protocol for GOLGA2/GM130 antibody 66662-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
humanWB

Mol Metab

CD36 promotes de novo lipogenesis in hepatocytes through INSIG2-dependent SREBP1 processing.

Authors - Han Zeng
human,ratWB

Front Mol Biosci

Schwann Cell-Derived Exosomes Induce the Differentiation of Human Adipose-Derived Stem Cells Into Schwann Cells.

Authors - Nan Zhou
humanIF

Exp Cell Res

NLRX1 and STING alleviate renal ischemia-reperfusion injury by regulating LC3 lipidation during mitophagy

Authors - Yinping Liao
mouseWB

STAR Protoc

Protocol for extraction of gut interstitial fluid in mice with two-front nutrient supply

Authors - Jian Zhang
humanWB

Gene

Autocrine small extracellular vesicles induce tubular phenotypic transformation in diabetic nephropathy via miR-21-5p

Authors - Mengting Zhang
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