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  • Featured Product
  • KD/KO Validated

GM2A Monoclonal antibody, PBS Only

GM2A Monoclonal Antibody for ELISA, WB

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

1E4D3

Cat no : 66080-1-PBS

Print datasheet

Synonyms

SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short

验证数据展示

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

产品信息

66080-1-PBS targets GM2A in ELISA, WB applications and shows reactivity with human samples.

Tested Applications ELISA, WB Application Description
Tested Reactivity human
Immunogen GM2A fusion protein Ag4394 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GM2 ganglioside activator
Synonyms SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.