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GMEB2 Polyclonal antibody

GMEB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13314-1-AP

Print datasheet

Synonyms

P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2

验证数据展示

  • WB analysis of human placenta using 13314-1-AP

    WB analysis of human placenta using 13314-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 13314-1-AP

    IHC staining of human cervical cancer using 13314-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with GMEB2 antibody included
  • IHC staining of human cervical cancer using 13314-1-AP

    IHC staining of human cervical cancer using 13314-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with GMEB2 antibody included
  • IF Staining of MCF-7 using 13314-1-AP

    IF Staining of MCF-7 using 13314-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GMEB2 antibody (13314-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse ovary tissue, mouse thymus tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy GMEB2 Ready-To-Use IHC Kit. GMEB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13314-1-AP targets GMEB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GMEB2 fusion protein Ag4157 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glucocorticoid modulatory element binding protein 2
Synonyms P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2
Calculated Molecular Weight 530 aa, 56 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC036305
Gene Symbol GMEB2
Gene ID (NCBI) 26205
RRIDAB_2110829
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMEB2, also named as Glucocorticoid modulatory element-binding protein 2, is a 530 amino acid protein, which is expressed in peripheral blood lymphocytes and fetal liver. GMEB2 as a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. The calcualted molecular weight of GMEB2 is 56 kDa, but modified GMEB2 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for GMEB2 antibody 13314-1-APDownload protocol
IHC protocol for GMEB2 antibody 13314-1-APDownload protocol
IF protocol for GMEB2 antibody 13314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols