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Geminin Monoclonal antibody, PBS Only

Geminin Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3A2

Cat no : 66566-1-PBS

Print datasheet

Synonyms

GMNN, MGORS6, Gem, 2E3A2

验证数据展示

  • WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

产品信息

66566-1-PBS targets Geminin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Geminin fusion protein Ag24283 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name geminin, DNA replication inhibitor
Synonyms GMNN, MGORS6, Gem, 2E3A2
Calculated Molecular Weight 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_2881927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75496
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)