验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells, Jurkat cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 6 publications below |
产品信息
15517-1-AP targets GMPPA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | GMPPA fusion protein Ag7789 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | GDP-mannose pyrophosphorylase A |
别名 | Mannose-1-phosphate guanyltransferase alpha, GTP-mannose-1-phosphate guanylyltransferase alpha, GMPP-alpha, GDP-mannose pyrophosphorylase A, GDP mannose pyrophosphorylase A |
计算分子量 | 46 kDa |
观测分子量 | 46 kDa |
GenBank蛋白编号 | BC007456 |
基因名称 | GMPPA |
Gene ID (NCBI) | 29926 |
RRID | AB_2111056 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96IJ6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for GMPPA antibody 15517-1-AP | Download protocol |
IHC protocol for GMPPA antibody 15517-1-AP | Download protocol |
IP protocol for GMPPA antibody 15517-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
iScience A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation. | ||
Front Nutr Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12 | ||
Biomedicines Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex
| ||
Front Mol Neurosci Ubiquitination contributes to the regulation of GDP-mannose pyrophosphorylase B activity | ||
J Clin Invest GMPPA defects cause a neuromuscular disorder with α-dystroglycan hyperglycosylation |