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GMPPA Polyclonal antibody

GMPPA Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15517-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

Mannose-1-phosphate guanyltransferase alpha, GTP-mannose-1-phosphate guanylyltransferase alpha, GMPP-alpha, GDP-mannose pyrophosphorylase A, GDP mannose pyrophosphorylase A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15517-1-AP targets GMPPA in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 GMPPA fusion protein Ag7789 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GDP-mannose pyrophosphorylase A
别名 Mannose-1-phosphate guanyltransferase alpha, GTP-mannose-1-phosphate guanylyltransferase alpha, GMPP-alpha, GDP-mannose pyrophosphorylase A, GDP mannose pyrophosphorylase A
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC007456
基因名称 GMPPA
Gene ID (NCBI) 29926
RRIDAB_2111056
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GMPPA antibody 15517-1-APDownload protocol
IHC protocol for GMPPA antibody 15517-1-APDownload protocol
IP protocol for GMPPA antibody 15517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.

Authors - Norio Sasaoka
mouseWB

Front Mol Biosci

Altered Glycosylation in the Aging Heart.

Authors - Patricia Franzka
human,mouseWB

Front Nutr

Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12

Authors - Patricia Franzka
mouseWB

Biomedicines

Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex

Authors - Patricia Franzka
  • KO Validated
humanWB

Front Mol Neurosci

Ubiquitination contributes to the regulation of GDP-mannose pyrophosphorylase B activity

Authors - Patricia Franzka
mouseWB

J Clin Invest

GMPPA defects cause a neuromuscular disorder with α-dystroglycan hyperglycosylation

Authors - Patricia Franzka
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