验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, fetal human brain tissue, rat brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | human kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GNAI1 Ready-To-Use IHC Kit. GNAI1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12617-1-AP targets GNAI1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GNAI1 fusion protein Ag3290 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1 |
| 别名 | Gi, GNAI1 |
| 计算分子量 | 354 aa, 40 kDa |
| 观测分子量 | 38-40 kDa |
| GenBank蛋白编号 | BC026326 |
| 基因名称 | GNAI1 |
| Gene ID (NCBI) | 2770 |
| RRID | AB_2111346 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P63096 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAI1 antibody 12617-1-AP | Download protocol |
| IHC protocol for GNAI1 antibody 12617-1-AP | Download protocol |
| IP protocol for GNAI1 antibody 12617-1-AP | Download protocol |
| FC protocol for GNAI1 antibody 12617-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Drug Des Devel Ther Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence. | ||
J Oncol Characterization of the Newly Established Homoharringtonine- (HHT-) Resistant Cell Lines and Mechanisms of Resistance |
