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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 68266-1-Ig

    WB analysis using 68266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

GNAI1 Monoclonal antibody

GNAI1 Monoclonal Antibody for WB, ELISA
Cat No. 68266-1-Ig
CloneNo. 1G11H5

Host / Isotype

Mouse / IgG2b

Reactivity

Mouse, Rat, Pig, Chicken

Applications

WB, ELISA

Synonyms

Gi, GNAI1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, pig brain tissue, chicken brain tissue, mouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy GNAI1 Ready-To-Use IHC Kit. GNAI1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68266-1-Ig targets GNAI1 in WB, ELISA applications and shows reactivity with Mouse, Rat, Pig, Chicken samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Mouse, Rat, Pig, Chicken
Immunogen GNAI1 fusion protein Ag3290 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Synonyms Gi, GNAI1
Calculated Molecular Weight 354 aa, 40 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC026326
Gene Symbol GNAI1
Gene ID (NCBI) 2770
RRIDAB_2935351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63096
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for GNAI1 antibody 68266-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols