验证数据展示
经过测试的应用
Positive WB detected in | PC-12 cells, HepG2 cells, mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27264-1-AP targets GNAQ in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | GNAQ fusion protein Ag26170 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | guanine nucleotide binding protein (G protein), q polypeptide |
别名 | G ALPHA q, GAQ, GNAQ |
计算分子量 | 42 kDa |
观测分子量 | 38-40 kDa |
GenBank蛋白编号 | BC057777 |
基因名称 | GNAQ |
Gene ID (NCBI) | 2776 |
RRID | AB_2880822 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P50148 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)
实验方案
Product Specific Protocols | |
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WB protocol for GNAQ antibody 27264-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |