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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of PC-12 using 27264-1-AP

    WB analysis of PC-12 using 27264-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27264-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GNAQ Polyclonal antibody

GNAQ Polyclonal Antibody for WB, ELISA
Cat No. 27264-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

G ALPHA q, GAQ, GNAQ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-12 cells, HepG2 cells, mouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27264-1-AP targets GNAQ in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GNAQ fusion protein Ag26170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), q polypeptide
Synonyms G ALPHA q, GAQ, GNAQ
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC057777
Gene Symbol GNAQ
Gene ID (NCBI) 2776
RRIDAB_2880822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

实验方案

Product Specific Protocols
WB protocol for GNAQ antibody 27264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols