GNAQ Monoclonal antibody
GNAQ Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat, Rabbit, Pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2H5G6
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, HeLa cells, pig brain tissue, rabbit brain tissue, mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68191-1-Ig targets GNAQ in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, Mouse, Rat, Rabbit, Pig |
| Immunogen | GNAQ fusion protein Ag26178 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | guanine nucleotide binding protein (G protein), q polypeptide |
| Synonyms | G ALPHA q, GAQ, GNAQ |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC057777 |
| Gene Symbol | GNAQ |
| Gene ID (NCBI) | 2776 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAQ antibody 68191-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
