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GNB1 Polyclonal antibody
GNB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10247-2-AP targets GNB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | GNB1 fusion protein Ag0313 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | guanine nucleotide binding protein (G protein), beta polypeptide 1 |
| Synonyms | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, GBB1 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC004186 |
| Gene Symbol | GNB1 |
| Gene ID (NCBI) | 2782 |
| RRID | AB_2111820 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62873 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNB1(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1) belongs to the WD repeat G protein beta family.The guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems and the beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.GNB1 can form dimers with all gamma subunits analyzed(PMID:12782285).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNB1 antibody 10247-2-AP | Download protocol |
| IHC protocol for GNB1 antibody 10247-2-AP | Download protocol |
| IF protocol for GNB1 antibody 10247-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Pathog H9N2 virus-derived M1 protein promotes H5N6 virus release in mammalian cells: Mechanism of avian influenza virus inter-species infection in humans. | ||
Neuropharmacology. A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission. | ||
Exp Eye Res Nr2e3-directed transcriptional regulation of genes involved in photoreceptor development and cell-type specific phototransduction. | ||
Dev Dyn Neural tube defects and impaired neural progenitor cell proliferation in Gbeta1-deficient mice. | ||
Bioengineered Shikonin induces cell autophagy via modulating the microRNA -545-3p/guanine nucleotide binding protein beta polypeptide 1 axis, thereby disrupting cellular carcinogenesis in colon cancer. |
