GNB3 Polyclonal antibody

GNB3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10081-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, mouse embryo tissue, rat brain tissue, mouse cerebellum tissue, mouse eye tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10081-1-AP targets GNB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebrafish
免疫原 GNB3 fusion protein Ag0127 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 3
别名 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
计算分子量 37 kDa
观测分子量 35-37 kDa
GenBank蛋白编号BC000115
基因名称 GNB3
Gene ID (NCBI) 2784
RRIDAB_2263264
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
WB protocol for GNB3 antibody 10081-1-APDownload protocol
IHC protocol for GNB3 antibody 10081-1-APDownload protocol
IF protocol for GNB3 antibody 10081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neuron

Sustained antidepressant effects of ketamine metabolite involve GABAergic inhibition-mediated molecular dynamics in aPVT glutamatergic neurons

Authors - Ayako Kawatake-Kuno
mouseIF

Elife

Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair

Authors - Justinn Barr
zebrafishWB

Hum Mol Genet

CERKL gene knockout disturbs photoreceptor outer segment phagocytosis and causes rod-cone dystrophy in zebrafish.

Authors - Shanshan Yu
zebrafishWB

J Biol Chem

Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.

Authors - Fei Liu
ratWB

Sci Rep

PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.

Authors - Jing Li
humanIF

Nat Commun

The immune landscape of human thymic epithelial tumors

Authors - Zhongwei Xin
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