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验证数据展示

  • WB analysis using 11978-2-AP

    WB analysis using 11978-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11978-2-AP (GNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 11978-2-AP

    IP experiment of mouse brain using 11978-2-AP

    IP result of anti-GNB4 (IP:11978-2-AP, 4ug; Detection:11978-2-AP 1:800) with mouse brain tissue lysate 4000ug.

  • IF Staining of HepG2 using 11978-2-AP

    IF Staining of HepG2 using 11978-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB4 antibody (11978-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of SH-SY5Y using 11978-2-AP

    IF Staining of SH-SY5Y using 11978-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNB4 antibody (11978-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GNB4 Polyclonal antibody

GNB4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 11978-2-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

HG2B, Guanine nucleotide-binding protein subunit beta-4, CMTD1F

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, MCF-7 cells, SH-SY5Y cells, human placenta tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11978-2-AP targets GNB4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 GNB4 fusion protein Ag2604 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), beta polypeptide 4
别名 HG2B, Guanine nucleotide-binding protein subunit beta-4, CMTD1F
计算分子量 340 aa, 38 kDa
观测分子量 38 kDa
GenBank蛋白编号BC000873
基因名称 GNB4
Gene ID (NCBI) 59345
RRIDAB_2109620
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HAV0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G protein subunit beta 4 (GNB4), is one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins. GNB4 is important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

实验方案

Product Specific Protocols
WB protocol for GNB4 antibody 11978-2-APDownload protocol
IF protocol for GNB4 antibody 11978-2-APDownload protocol
IP protocol for GNB4 antibody 11978-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

BMC Med

Helicobacter pylori-induced aberrant demethylation and expression of GNB4 promotes gastric carcinogenesis via the Hippo-YAP1 pathway

Authors - Duanrui Liu
View Article
humanWB

Nat Commun

Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo

Authors - Jasjot Singh
View Article