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GNB5 Polyclonal antibody
GNB5 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue |
Positive IP detected in | rat brain tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11045-2-AP targets GNB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | GNB5 fusion protein Ag1518 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | guanine nucleotide binding protein (G protein), beta 5 |
Synonyms | GB5, Gbeta5, GNB5, Transducin beta chain 5 |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 39-42 kDa |
GenBank Accession Number | BC011671 |
Gene Symbol | GNB5 |
Gene ID (NCBI) | 10681 |
RRID | AB_2109639 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14775 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GNB5 antibody 11045-2-AP | Download protocol |
IHC protocol for GNB5 antibody 11045-2-AP | Download protocol |
IP protocol for GNB5 antibody 11045-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogene The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.
| ||
J Cell Mol Med Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring. | ||
Neuropharmacology. A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission. | ||
PMC Biophys Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling. | ||
MedComm (2020) Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing |