验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, human brain tissue |
Positive IP detected in | rat brain tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11045-2-AP targets GNB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | GNB5 fusion protein Ag1518 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | guanine nucleotide binding protein (G protein), beta 5 |
别名 | GB5, Gbeta5, GNB5, Transducin beta chain 5 |
计算分子量 | 39 kDa |
观测分子量 | 39-42 kDa |
GenBank蛋白编号 | BC011671 |
基因名称 | GNB5 |
Gene ID (NCBI) | 10681 |
RRID | AB_2109639 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O14775 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.
实验方案
Product Specific Protocols | |
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WB protocol for GNB5 antibody 11045-2-AP | Download protocol |
IHC protocol for GNB5 antibody 11045-2-AP | Download protocol |
IP protocol for GNB5 antibody 11045-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Oncogene The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.
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J Cell Mol Med Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring. | ||
Neuropharmacology. A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission. | ||
PMC Biophys Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling. | ||
MedComm (2020) Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing |