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  • KD/KO Validated

GNB5 Polyclonal antibody

GNB5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11045-2-AP

Print datasheet

Synonyms

GB5, Gbeta5, GNB5, Transducin beta chain 5



经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11045-2-AP targets GNB5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen GNB5 fusion protein Ag1518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), beta 5
Synonyms GB5, Gbeta5, GNB5, Transducin beta chain 5
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC011671
Gene Symbol GNB5
Gene ID (NCBI) 10681
RRIDAB_2109639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14775
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.

实验方案

Product Specific Protocols
WB protocol for GNB5 antibody 11045-2-APDownload protocol
IHC protocol for GNB5 antibody 11045-2-APDownload protocol
IP protocol for GNB5 antibody 11045-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

The Gβ5 protein regulates sensitivity to TRAIL-induced cell death in colon carcinoma.

Authors - D Fuchs
  • KD Validated
ratWB

J Cell Mol Med

Prenatal hypoxia inhibited propionate-evoked BK channels of mesenteric artery smooth muscle cells in offspring.

Authors - Wenna Zhang
mouseWB

Neuropharmacology.

A systems genetic analysis of alcohol drinking by mice, rats and men: Influence of brain GABAergic transmission.

Authors - Saba LM
mouseWB, IF

PMC Biophys

Retinal cone photoreceptors require phosducin-like protein 1 for g protein complex assembly and signaling.

Authors - Christopher M Tracy
humanWB

FEBS J

PMID: 30719834

Authors - Sang-Min Park
  • KD Validated
ratWB

MedComm (2020)

Transcriptomic analysis of rat brain response to alternating current electrical stimulation: unveiling insights via single-nucleus RNA sequencing

Authors - Yan Wang