验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, Jurkat cells, RAW 264.7 cells, rat liver tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human kidney tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25079-1-AP targets GNE in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GNE fusion protein Ag18689 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase |
| 别名 | N-acetylmannosamine kinase, ManAc kinase, IBM2, hydrolyzing, GLCNE |
| 计算分子量 | 722 aa, 79 kDa |
| 观测分子量 | 79 kDa |
| GenBank蛋白编号 | BC121179 |
| 基因名称 | GNE |
| Gene ID (NCBI) | 10020 |
| RRID | AB_2879887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y223 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNE antibody 25079-1-AP | Download protocol |
| IHC protocol for GNE antibody 25079-1-AP | Download protocol |
| IP protocol for GNE antibody 25079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neoplasma miR-550a-5p promotes the proliferation and migration of hepatocellular carcinoma by targeting GNE via the Wnt/β-catenin signaling pathway | ||
Cell Rep Efficient generation of isogenic primary human myeloid cells using CRISPR-Cas9 ribonucleoproteins. | ||
