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验证数据展示

  • WB analysis of mouse eye using 11884-1-AP

    WB analysis of mouse eye using 11884-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 11884-1-AP (GNGT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11884-1-AP

    IF Staining of HeLa using 11884-1-AP

    Immunofluorescent analysis of HeLa cells using 11884-1-AP (GNGT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GNGT1 Polyclonal antibody

GNGT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11884-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Transducin gamma chain, Guanine nucleotide-binding protein G(T) subunit gamma-T1, GNG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11884-1-AP targets GNGT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GNGT1 fusion protein Ag2488 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
别名 Transducin gamma chain, Guanine nucleotide-binding protein G(T) subunit gamma-T1, GNG1
计算分子量 74 aa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC029367
基因名称 GNGT1
Gene ID (NCBI) 2792
RRIDAB_10858628
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP63211
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

G protein subunit gamma-T1(GNGT1) is encoded by the transducin gamma-subunit gene, which localized on huaman chromosome 7q21.3, participating in various transmembrane signaling systems. Particularly, GNGT1 gene is specific to retinal rod photoreceptors. Present polyclonal anti-GNGT1 antibody (11884-1-AP) is produced by immunizing rabbits with full-length chain of GNGT1 and dectects an 8 kDa band in mouse retinal tissue.

实验方案

Product Specific Protocols
WB protocol for GNGT1 antibody 11884-1-APDownload protocol
IF protocol for GNGT1 antibody 11884-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols