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GNGT2 Polyclonal antibody

GNGT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11988-1-AP

Print datasheet

Synonyms

GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8

验证数据展示

  • WB analysis using 11988-1-AP

    WB analysis using 11988-1-AP

    Mouse retina tissue were subjected to SDS PAGE followed by western blot with 11988-1-AP (GNGT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 11988-1-AP

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 11988-1-AP

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11988-1-AP targets GNGT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GNGT2 fusion protein Ag2618 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 2
Synonyms GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8
Calculated Molecular Weight 69 aa, 8 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC008663
Gene Symbol GNGT2
Gene ID (NCBI) 2793
RRIDAB_2877813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14610
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNGT2, also known as GNG8, GNG9 and GNGT8, belongs to the G protein gamma family. Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. This antibody has weak cross-reactivity of GNGT1 protein, as the immunogen of GNGT2 shares about 65% homology with GNGT1.

实验方案

Product Specific Protocols
WB protocol for GNGT2 antibody 11988-1-APDownload protocol
IHC protocol for GNGT2 antibody 11988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols