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验证数据展示

  • WB analysis using 11988-1-AP

    WB analysis using 11988-1-AP

    Mouse retina tissue were subjected to SDS PAGE followed by western blot with 11988-1-AP (GNGT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 11988-1-AP

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse eye using 11988-1-AP

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GNGT2 Polyclonal antibody

GNGT2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11988-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11988-1-AP targets GNGT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 GNGT2 fusion protein Ag2618 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 2
别名 GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8
计算分子量 69 aa, 8 kDa
观测分子量 8 kDa
GenBank蛋白编号BC008663
基因名称 GNGT2
Gene ID (NCBI) 2793
RRIDAB_2877813
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNGT2, also known as GNG8, GNG9 and GNGT8, belongs to the G protein gamma family. Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. This antibody has weak cross-reactivity of GNGT1 protein, as the immunogen of GNGT2 shares about 65% homology with GNGT1.

实验方案

Product Specific Protocols
WB protocol for GNGT2 antibody 11988-1-APDownload protocol
IHC protocol for GNGT2 antibody 11988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Toxicology

C-C motif chemokine ligand 5 contributes to radon exposure-induced lung injury by recruiting dendritic cells to activate effector T helper cells

Authors - Liping Ma
View Article