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GNL1 Polyclonal antibody

GNL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14078-1-AP

Print datasheet

Synonyms

HSR1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1

验证数据展示

  • WB analysis using 14078-1-AP

    WB analysis using 14078-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 14078-1-AP

    IHC staining of human heart using 14078-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 14078-1-AP

    IHC staining of human heart using 14078-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of MCF-7 using 14078-1-AP

    IF Staining of MCF-7 using 14078-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNL1 antibody (14078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, Raji cells, U-937 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman heart tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14078-1-AP targets GNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GNL1 fusion protein Ag5203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein-like 1
Synonyms HSR1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1
Calculated Molecular Weight 607 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC018366
Gene Symbol GNL1
Gene ID (NCBI) 2794
RRIDAB_10641989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36915
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide-binding protein-like 1 (GNL1), also known as HSR1, belongs to HSR1_MMR1 subfamily of nucleolar GTPases. It encodes 607 amino acids with a molecular mass of 69 kDa and contains basic amino acids rich N-terminus, acidic amino acids rich C-terminus and proline rich-domains. GNL1 plays a critical role in liver cell proliferation, but its function remains largely unknown (PMID: 18255255).

实验方案

Product Specific Protocols
WB protocol for GNL1 antibody 14078-1-APDownload protocol
IHC protocol for GNL1 antibody 14078-1-APDownload protocol
IF protocol for GNL1 antibody 14078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.

Authors - Zewei Tu
View Article