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验证数据展示

  • WB analysis using 10190-2-AP

    WB analysis using 10190-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10190-2-AP (GNL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10190-2-AP

    IHC staining of human breast cancer using 10190-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10190-2-AP (GNL2 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of A431 using 10190-2-AP

    IF Staining of A431 using 10190-2-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using GNL2 antibody (10190-2-AP) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

GNL2 Polyclonal antibody

GNL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10190-2-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nucleolar GTP-binding protein 2, NGP1, Ngp 1, HUMAUANTIG, Autoantigen NGP-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Ramos cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10190-2-AP targets GNL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GNL2 fusion protein Ag0243 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name guanine nucleotide binding protein-like 2 (nucleolar)
Synonyms Nucleolar GTP-binding protein 2, NGP1, Ngp 1, HUMAUANTIG, Autoantigen NGP-1
Calculated Molecular Weight 731 aa, 84 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC000107
Gene Symbol GNL2
Gene ID (NCBI) 29889
RRIDAB_2110075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13823
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNL2 ( G protein nucleolar 2) has a crucial role in the pathogenesis of gliomas by influencing key cellular processes, particularly enhanced protein synthesis, which ultimately contributes to the development of gliomas. In addition, GNL2 was found to affect the overall protein synthesis of ribosomal protein L11 in glioma cells. The calculated MW of GNL2 is 84 kDa, but the observed MW is around 100 kDa, which may be due to glycosylation modification.

实验方案

Product Specific Protocols
WB protocol for GNL2 antibody 10190-2-APDownload protocol
IHC protocol for GNL2 antibody 10190-2-APDownload protocol
IF protocol for GNL2 antibody 10190-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols