验证数据展示
经过测试的应用
Positive WB detected in | rat liver tissue, mouse liver tissue, LNCaP cells |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | human prostate hyperplasia tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 27 publications below |
IHC | See 2 publications below |
产品信息
18790-1-AP targets GNMT in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig, chicken |
免疫原 | GNMT fusion protein Ag4598 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glycine N-methyltransferase |
别名 | glycine N methyltransferase, GNMT |
计算分子量 | 295 aa, 33 kDa |
观测分子量 | 33 kDa |
GenBank蛋白编号 | BC032627 |
基因名称 | GNMT |
Gene ID (NCBI) | 27232 |
RRID | AB_10597093 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14749 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).
实验方案
Product Specific Protocols | |
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WB protocol for GNMT antibody 18790-1-AP | Download protocol |
IHC protocol for GNMT antibody 18790-1-AP | Download protocol |
IP protocol for GNMT antibody 18790-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Metab Disrupted Liver Oxidative Metabolism in Glycine N-Methyltransferase-Deficient Mice is Mitigated by Dietary Methionine Restriction. | ||
Int J Mol Sci MAT2A Localization and Its Independently Prognostic Relevance in Breast Cancer Patients. | ||
J Anim Sci Biotechnol Dietary betaine activates hepaticVTGIIexpression in laying hens associated with hypomethylation ofGRgene promoter and enhancedGRexpression. | ||
Mol Oncol Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform. | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. | ||
J Agric Food Chem Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. |