验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human placenta tissue, Transfected HEK-293 cells, COLO 320 cells, HeLa cells, PC-3 cells, mouse brain tissue |
| Positive IP detected in | COLO 320 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14931-1-AP targets GNPAT in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, hamster |
| 免疫原 | GNPAT fusion protein Ag6735 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glyceronephosphate O-acyltransferase |
| 别名 | DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT |
| 计算分子量 | 77 kDa |
| 观测分子量 | 65-69 kDa |
| GenBank蛋白编号 | BC000450 |
| 基因名称 | GNPAT |
| Gene ID (NCBI) | 8443 |
| RRID | AB_2110546 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15228 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID:21990100) This antibody recognize the 69 kDa GNPAT protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNPAT antibody 14931-1-AP | Download protocol |
| IP protocol for GNPAT antibody 14931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Functional multi-organelle units control inflammatory lipid metabolism of macrophages | ||
Cell Host Microbe Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.
| ||
J Clin Invest Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission. | ||
Int J Mol Sci Differential Expression of Peroxisomal Proteins in Distinct Types of Parotid Gland Tumors. | ||
Hum Mutat Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3. | ||
Front Cell Dev Biol Plasmalogens Regulate Retinal Connexin 43 Expression and Müller Glial Cells Gap Junction Intercellular Communication and Migration.
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