验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse large intestine tissue, MDA-MB-231 cells, mouse colon tissue |
| Positive IP detected in | mouse large intestine tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16282-1-AP targets GNPNAT1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GNPNAT1 fusion protein Ag9375 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glucosamine-phosphate N-acetyltransferase 1 |
| 别名 | GNA1, GNPNAT, GNPNAT1, Gpnat1, Phosphoglucosamine acetylase |
| 计算分子量 | 184 aa, 21 kDa |
| 观测分子量 | 21-23 kDa |
| GenBank蛋白编号 | BC012179 |
| 基因名称 | GNPNAT1 |
| Gene ID (NCBI) | 64841 |
| RRID | AB_2110243 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96EK6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glucosamine-6 phosphate-N-acetyl Transferase (GNPNAT1) is a key enzyme in the hexosamine biosynthetic pathway. It converts D-glucosamine 6-phosphate to N-acetyl-D-glucosamine 6-phosphate, the acetylation step allowing carbon and nitrogen enter into the hexosamine biosynthetic pathway. Recently inhibition of GNPNAT1 has been reported to promote proliferation and aggressiveness of castration-resistant prostate cancer. (27194471) GNPNAT1 is a small dimeric protein localized in the Golgi and endosome membranes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GNPNAT1 antibody 16282-1-AP | Download protocol |
| IHC protocol for GNPNAT1 antibody 16282-1-AP | Download protocol |
| IP protocol for GNPNAT1 antibody 16282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Nat Commun Inhibition of the hexosamine biosynthetic pathway promotes castration-resistant prostate cancer.
| ||
Front Immunol Risk stratification based on DNA damage-repair-related signature reflects the microenvironmental feature, metabolic status and therapeutic response of breast cancer | ||
Front Mol Biosci Upregulation of GNPNAT1 Predicts Poor Prognosis and Correlates With Immune Infiltration in Lung Adenocarcinoma. | ||
Front Immunol GNPNAT1 is a potential biomarker correlated with immune infiltration and immunotherapy outcome in breast cancer | ||
Int J Mol Sci ER Stress-Perturbed Intracellular Protein O-GlcNAcylation Aggravates Podocyte Injury in Diabetes Nephropathy |
