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GNRHR Polyclonal antibody, PBS Only

GNRHR Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 19950-1-PBS

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Synonyms

验证数据展示

  • FC experiment of MCF-7 using 19950-1-AP (same clone as 19950-1-PBS)

    FC experiment of MCF-7 using 19950-1-AP (same clone as 19950-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human GNRHR (19950-1-AP) (red) or 0.2 ug Control Antibody (blue), and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • WB analysis using 19950-1-AP (same clone as 19950-1-PBS)

    WB analysis using 19950-1-AP (same clone as 19950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19950-1-AP (GNRHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

    IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

    IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

产品信息

19950-1-PBS targets GNRHR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GnRH receptor
Synonyms
Calculated Molecular Weight 38 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberNM_000406
Gene Symbol GNRHR
Gene ID (NCBI) 2798
RRIDAB_10644155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30968
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.