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验证数据展示

  • WB analysis of mouse ovary using 22462-1-AP

    WB analysis of mouse ovary using 22462-1-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22462-1-AP (GNRHR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22462-1-AP

    IHC staining of human testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 22462-1-AP

    IHC staining of human testis using 22462-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 22462-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens).

GNRHR Polyclonal antibody

GNRHR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22462-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

GnRH R, GnRH receptor, GNRHR, GNRHR1, GRHR, LHRHR, LRHR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse ovary tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, rat testis tissue, mouse testis tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22462-1-AP targets GNRHR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GNRHR fusion protein Ag18005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GnRH receptor
别名 GnRH R, GnRH receptor, GNRHR, GNRHR1, GRHR, LHRHR, LRHR
计算分子量 328 aa, 38 kDa
观测分子量 60 kDa, 50 kDa
GenBank蛋白编号BC113546
基因名称 GNRHR
Gene ID (NCBI) 2798
RRIDAB_2879109
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30968
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.

实验方案

Product Specific Protocols
WB protocol for GNRHR antibody 22462-1-APDownload protocol
IHC protocol for GNRHR antibody 22462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols