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验证数据展示

  • WB analysis of human brain using 20728-1-AP

    WB analysis of human brain using 20728-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20728-1-AP (GNRHR2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20728-1-AP

    IHC staining of human kidney using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20728-1-AP (GNRHR2 Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 20728-1-AP

    IF Staining of HEK-293 using 20728-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20728-1-AP (GNRHR2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human liver cancer using 20728-1-AP

    IHC staining of human liver cancer using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 20728-1-AP

    IHC staining of human liver cancer using 20728-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20728-1-AP (GNRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GNRHR2 Polyclonal antibody

GNRHR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20728-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GnRH II R, GNRHR2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, human placenta tissue, human stomach tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20728-1-AP targets GNRHR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name gonadotropin-releasing hormone (type 2) receptor 2
Synonyms GnRH II R, GNRHR2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberNR_002328
Gene Symbol GNRHR2
Gene ID (NCBI) 114814
RRIDAB_2878728
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GNRHR2 antibody 20728-1-APDownload protocol
IHC protocol for GNRHR2 antibody 20728-1-APDownload protocol
IF protocol for GNRHR2 antibody 20728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols