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验证数据展示

  • WB analysis using 31164-1-AP

    WB analysis using 31164-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31164-1-AP (GOLGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 31164-1-AP

    IF Staining of HepG2 using 31164-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA5 antibody (31164-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GOLGA5 Polyclonal antibody

GOLGA5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31164-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

PIG31, GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31164-1-AP targets GOLGA5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GOLGA5 fusion protein Ag35031 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 5
别名 PIG31, GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84
计算分子量 83 kDa
观测分子量 83-95 kDa
GenBank蛋白编号BC023021
基因名称 GOLGA5
Gene ID (NCBI) 9950
RRIDAB_3669878
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TBA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).

实验方案

Product Specific Protocols
WB protocol for GOLGA5 antibody 31164-1-APDownload protocol
IF protocol for GOLGA5 antibody 31164-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols