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验证数据展示

  • WB analysis using 83839-2-RR

    WB analysis using 83839-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83839-2-RR (GOLGA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 83839-2-RR

    IHC staining of human ovary cancer using 83839-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83839-2-RR (GOLGA5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83839-2-RR

    Affinity and Kinetic Characterization of 83839-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83839-2-RR against Human GOLGA5 were performed. The affinity constant is 5.79 nM.

GOLGA5 Recombinant antibody

GOLGA5 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83839-2-RR

产品说明书

CloneNo. 240893F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84, Cell proliferation-inducing gene 31 protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83839-2-RR targets GOLGA5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 GOLGA5 fusion protein Ag35031 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi autoantigen, golgin subfamily a, 5
别名 GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84, Cell proliferation-inducing gene 31 protein
计算分子量 83 kDa
观测分子量 80-95 kDa
GenBank蛋白编号BC023021
基因名称 GOLGA5
Gene ID (NCBI) 9950
RRIDAB_3671422
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TBA6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).

实验方案

Product Specific Protocols
WB protocol for GOLGA5 antibody 83839-2-RRDownload protocol
IHC protocol for GOLGA5 antibody 83839-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols