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GOLGA7 Polyclonal antibody

GOLGA7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14723-1-AP

Print datasheet

Synonyms

GCP16, GOLGA3AP1, GOLGA7, GOLGA7A, Golgin subfamily A member 7, HSPC041

验证数据展示

  • WB analysis using 14723-1-AP

    WB analysis using 14723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14723-1-AP (GOLGA7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 14723-1-AP

    WB analysis of HEK-293 using 14723-1-AP

    WB result of GOLGA7 antibody (14723-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA7 transfected HEK-293 cells.

  • IHC staining of mouse stomach using 14723-1-AP

    IHC staining of mouse stomach using 14723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 14723-1-AP

    IHC staining of mouse stomach using 14723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HL-60 cells, HepG2 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14723-1-AP targets GOLGA7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GOLGA7 fusion protein Ag6460 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi autoantigen, golgin subfamily a, 7
Synonyms GCP16, GOLGA3AP1, GOLGA7, GOLGA7A, Golgin subfamily A member 7, HSPC041
Calculated Molecular Weight 16 kDa
Observed Molecular Weight15-20 kDa
GenBank Accession NumberBC012032
Gene Symbol GOLGA7
Gene ID (NCBI) 51125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GOLGA7 (Golgin subfamily A member 7) is an acylated Golgi protein that interacts with GCP170 protein (PMID: 14522980). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. The ZDHHC5-GOLGA7 protein acyltransferase complex promotes nonapoptotic cell death (PMID: 31631010).

实验方案

Product Specific Protocols
WB protocol for GOLGA7 antibody 14723-1-APDownload protocol
IHC protocol for GOLGA7 antibody 14723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols