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  • Featured Product
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GOSR2/Membrin Polyclonal antibody

GOSR2/Membrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12095-1-AP

Print datasheet

Synonyms

Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein

验证数据展示

  • WB analysis of Jurkat using 12095-1-AP

    WB analysis of Jurkat using 12095-1-AP

    WB result of GOSR2/Membrin antibody (12095-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

  • WB analysis using 12095-1-AP

    WB analysis using 12095-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 12095-1-AP

    IF Staining of HeLa using 12095-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOSR2/Membrin antibody (12095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human stomach using 12095-1-AP

    IHC staining of human stomach using 12095-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, human liver tissue, mouse liver tissue, mouse stomach tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12095-1-AP targets GOSR2/Membrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen GOSR2/Membrin fusion protein Ag2736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi SNAP receptor complex member 2
Synonyms Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein
Calculated Molecular Weight 212 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC009710
Gene Symbol Membrin
Gene ID (NCBI) 9570
RRIDAB_2113610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

实验方案

Product Specific Protocols
WB protocol for GOSR2/Membrin antibody 12095-1-APDownload protocol
IHC protocol for GOSR2/Membrin antibody 12095-1-APDownload protocol
IF protocol for GOSR2/Membrin antibody 12095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Commun

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models

Authors - Ying Xue Xie
View Article
WB

Commun Biol

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Authors - Xihua Yue
View Article