- Featured Product
- KD/KO Validated
GOSR2/Membrin Monoclonal antibody
GOSR2/Membrin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
7E7C7
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, Jurkat cells, PC-12 cells, HEK-293 cells, HepG2 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66134-1-Ig targets GOSR2/Membrin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat |
| Cited Reactivity | human |
| Immunogen | GOSR2/Membrin fusion protein Ag21393 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | golgi SNAP receptor complex member 2 |
| Synonyms | 27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin |
| Calculated Molecular Weight | 212 aa, 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC009710 |
| Gene Symbol | Membrin |
| Gene ID (NCBI) | 9570 |
| RRID | AB_2881533 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GOSR2/Membrin antibody 66134-1-Ig | Download protocol |
| IHC protocol for GOSR2/Membrin antibody 66134-1-Ig | Download protocol |
| IF protocol for GOSR2/Membrin antibody 66134-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
