验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, mouse heart tissue, mouse spleen tissue, HEK-293 cells, mouse brain, rat brain |
| Positive IHC detected in | human breast cancer tissue, human skin tissue, rat brain tissue, human liver tissue, rat heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy GOT2 Ready-To-Use IHC Kit. GOT2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14800-1-AP targets GOT2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GOT2 fusion protein Ag6514 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) |
| 别名 | Fatty acid-binding protein, FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial |
| 计算分子量 | 47 kDa |
| 观测分子量 | 43-45 kDa |
| GenBank蛋白编号 | BC000525 |
| 基因名称 | GOT2 |
| Gene ID (NCBI) | 2806 |
| RRID | AB_2247898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00505 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GOT2 antibody 14800-1-AP | Download protocol |
| IHC protocol for GOT2 antibody 14800-1-AP | Download protocol |
| IF protocol for GOT2 antibody 14800-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.
| ||
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. | ||
Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
| ||
Nat Commun Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner. | ||
Nat Commun Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression. |
