验证数据展示
经过测试的应用
Positive WB detected in | Recombinant protein |
Positive IHC detected in | human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24494-1-AP targets GP repeat in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, ELISA |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | GP repeat |
别名 | C9RAN, GGGCC, GP repeat, GPGPGPGP, poly GP, poly PG |
基因名称 | |
Gene ID (NCBI) | |
RRID | AB_2879573 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The C9orf72 "GGGGCC" repeat sequence codes five repeat peptide "GA repeat; GAGAGAGAGA", "GP repeat; GPGPGPGPG", "GR repeat; GRGRGRGRG", "AP repeat; APAPAPAPA" and "PR repeat; PRPRPRPRP". It was described previously that aggregated forms of poly-GA and poly-GP proteins do not enter the separation gel (PMID: 26374446). This antibody is used to detect the GP repeated sequence. The recombinant protein 6*His tag-SMARCA4 or GST tag-SMARCA4 which contains the sequence "GPGPGPGPG" were used to detect this antibody.
实验方案
Product Specific Protocols | |
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WB protocol for GP repeat antibody 24494-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Neuropathol Quantitative analysis and clinico-pathological correlations of different dipeptide repeat protein pathologies in C9ORF72 mutation carriers. | ||
Acta Neuropathol Increased prevalence of granulovacuolar degeneration in C9orf72 mutation. | ||
Acta Neuropathol Antisense RNA foci in the motor neurons of C9ORF72-ALS patients are associated with TDP-43 proteinopathy. | ||
EMBO Rep Glucose hypometabolism prompts RAN translation and exacerbates C9orf72-related ALS/FTD phenotypes | ||
Neuropathol Appl Neurobiol Neurodegeneration in Frontotemporal Lobar Degeneration and Motor Neurone Disease associated with expansions in C9orf72 is linked to TDP-43 pathology and not associated with aggregated forms of dipeptide repeat proteins. | ||
Hum Mol Genet Modelling C9orf72 dipeptide repeat proteins of a physiologically relevant size. |