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验证数据展示

  • WB analysis using 66331-1-Ig (same clone as 66331-1-PBS)

    WB analysis using 66331-1-Ig (same clone as 66331-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66331-1-Ig (GP73/GOLPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

    IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

    IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IF Staining of HeLa using 66331-1-Ig (same clone as 66331-1-PBS)

    IF Staining of HeLa using 66331-1-Ig (same clone as 66331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (66331-1-Ig, Clone: 3D1F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51312-3

    Cytometric bead array standard curve of MP51312-3

    Cytometric bead array standard curve of MP51312-3, GOLM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66331-2-PBS. Detection antibody: 66331-1-PBS. Standard:Ag7207. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP51312-4

    Cytometric bead array standard curve of MP51312-4

    Cytometric bead array standard curve of MP51312-4, GOLM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66331-3-PBS. Detection antibody: 66331-1-PBS. Standard:Ag7207. Range: 0.098-100 ng/mL.

  • Featured Product
  • KD/KO验证

GP73/GOLPH2 Monoclonal antibody, PBS Only (Detector)

GP73/GOLPH2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66331-1-PBS

产品说明书

CloneNo. 3D1F1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

GOLM1, GOLPH2, GP73, golgi protein 73kD, Golgi phosphoprotein 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.

产品信息

66331-1-PBS targets GP73/GOLPH2 as part of a matched antibody pair:

MP51312-3: 66331-2-PBS capture and 66331-1-PBS detection (validated in Cytometric bead array)

MP51312-4: 66331-3-PBS capture and 66331-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 GP73/GOLPH2 fusion protein Ag7207 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi membrane protein 1
别名 GOLM1, GOLPH2, GP73, golgi protein 73kD, Golgi phosphoprotein 2
计算分子量 45 kDa
观测分子量 73 kDa
GenBank蛋白编号BC001740
基因名称 GP73
Gene ID (NCBI) 51280
RRIDAB_2881712
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NBJ4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.