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GP73/GOLPH2 Recombinant antibody, PBS Only

GP73/GOLPH2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I12

Cat no : 81893-1-PBS

Print datasheet

Synonyms

GOLM1, GOLPH2, GP73, golgi protein 73kD, Golgi phosphoprotein 2

验证数据展示

  • WB analysis using 81893-1-RR (same clone as 81893-1-PBS)

    WB analysis using 81893-1-RR (same clone as 81893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

    IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

    IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

    IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human stomach using 81893-1-RR (same clone as 81893-1-PBS)

    IHC staining of human stomach using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GOLM1 antibody included
  • Affinity and Kinetic Characterization of 81893-1-RR

    Affinity and Kinetic Characterization of 81893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.

产品信息

81893-1-PBS targets GP73/GOLPH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GP73/GOLPH2 fusion protein Ag7207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name golgi membrane protein 1
Synonyms GOLM1, GOLPH2, GP73, golgi protein 73kD, Golgi phosphoprotein 2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC001740
Gene Symbol GP73
Gene ID (NCBI) 51280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NBJ4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.