验证数据展示
经过测试的应用
Positive WB detected in | Human peripheral blood platelets |
Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
14564-1-AP targets CD42a / GP9 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human |
文献引用反应性 | mouse |
免疫原 | CD42a / GP9 fusion protein Ag6129 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glycoprotein IX (platelet) |
别名 | CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX |
计算分子量 | 19 kDa |
观测分子量 | 20 kDa |
GenBank蛋白编号 | BC030229 |
基因名称 | CD42a |
Gene ID (NCBI) | 2815 |
RRID | AB_2878067 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P14770 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.
实验方案
Product Specific Protocols | |
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WB protocol for CD42a / GP9 antibody 14564-1-AP | Download protocol |
IHC protocol for CD42a / GP9 antibody 14564-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |