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验证数据展示

  • WB analysis of human peripheral blood platelets using 14564-1-AP

    WB analysis of human peripheral blood platelets using 14564-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 14564-1-AP (CD42a / GP9 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 14564-1-AP

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 14564-1-AP

    IHC staining of human spleen using 14564-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14564-1-AP (CD42a / GP9 Antibody) at dilution of 1:50 (under 40x lens).

CD42a / GP9 Polyclonal antibody

CD42a / GP9 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14564-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA and More (1)

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHuman peripheral blood platelets
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14564-1-AP targets CD42a / GP9 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivitymouse
Immunogen CD42a / GP9 fusion protein Ag6129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glycoprotein IX (platelet)
Synonyms CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC030229
Gene Symbol CD42a
Gene ID (NCBI) 2815
RRIDAB_2878067
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14770
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

实验方案

Product Specific Protocols
WB protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
IHC protocol for CD42a / GP9 antibody 14564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Cell Death Dis

Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression

Authors - Rong Yan
View Article