验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10104-1-AP targets GPAA1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GPAA1 fusion protein Ag0152 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) |
| 别名 | GAA1, GAA1 protein homolog, GPAA1, hGAA1 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 60-68 kDa |
| GenBank蛋白编号 | BC004129 |
| 基因名称 | GPAA1 |
| Gene ID (NCBI) | 8733 |
| RRID | AB_2263708 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43292 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GPAA1(Glycosylphosphatidylinositol anchor attachment 1 protein) is also named as GAA1. It is the most conspicuously hydrophobic of the known subunits of GPIT, and it may play a key role in substrate recognition. It is predicted to be a multispanning membrane protein of 67 kDa with seven transmembrane (TM) domains, a large lumenal domain between the first two TM segments, and a cytoplasmically oriented N terminus bearing a potential ER retrieval signal in the form of a diarginine motif(PMID:12052837). It has 2 isoforms produced by alternative splicing and the full length protein has 2 glycosylation sites. This antibody is specific to GPAA1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPAA1 antibody 10104-1-AP | Download protocol |
| IHC protocol for GPAA1 antibody 10104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mod Pathol Profiling the expression pattern of GPI transamidase complex subunits in human cancer. | ||
Nat Commun CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection | ||
Cell Rep Targeting the GPI transamidase subunit GPAA1 abrogates the CD24 immune checkpoint in ovarian cancer
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