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验证数据展示

  • WB analysis of mouse spleen using 24366-1-AP

    WB analysis of mouse spleen using 24366-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24366-1-AP (GPATCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24366-1-AP

    IHC staining of human breast cancer using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 24366-1-AP

    IHC staining of human testis using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

GPATCH2 Polyclonal antibody

GPATCH2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24366-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

G patch domain containing 2, GPATC2, GPATCH2, RP11 361K17.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, mouse kidney tissue, mouse thymus tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24366-1-AP targets GPATCH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 GPATCH2 fusion protein Ag21647 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G patch domain containing 2
别名 G patch domain containing 2, GPATC2, GPATCH2, RP11 361K17.1
计算分子量 528 aa, 59 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC063474
基因名称 GPATCH2
Gene ID (NCBI) 55105
RRIDAB_2879508
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NW75
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPATCH2 antibody 24366-1-APDownload protocol
IHC protocol for GPATCH2 antibody 24366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Deletion of Gpatch2 does not alter Tnf expression in mice

Authors - Destiny Dalseno
View Article