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GPATCH2 Polyclonal antibody

GPATCH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24366-1-AP

Print datasheet

Synonyms

G patch domain containing 2, GPATC2, GPATCH2, RP11 361K17.1

验证数据展示

  • WB analysis of mouse spleen using 24366-1-AP

    WB analysis of mouse spleen using 24366-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24366-1-AP (GPATCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 24366-1-AP

    IHC staining of human breast cancer using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human testis using 24366-1-AP

    IHC staining of human testis using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse spleen tissue, mouse kidney tissue, mouse thymus tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 24366-1-AP is GPATCH2 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen GPATCH2 fusion protein Ag21647 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G patch domain containing 2
Synonyms G patch domain containing 2, GPATC2, GPATCH2, RP11 361K17.1
Calculated Molecular Weight 528 aa, 59 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC063474
Gene Symbol GPATCH2
Gene ID (NCBI) 55105
RRIDAB_2879508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NW75
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GPATCH2 antibody 24366-1-APDownload protocol
IHC protocol for GPATCH2 antibody 24366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Deletion of Gpatch2 does not alter Tnf expression in mice

Authors - Destiny Dalseno
View Article