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验证数据展示

  • WB analysis of HEK-293 using 24399-1-AP

    WB analysis of HEK-293 using 24399-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24399-1-AP

    IHC staining of human liver using 24399-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 24399-1-AP

    IHC staining of human liver cancer using 24399-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 24399-1-AP

    IF Staining of HEK-293 using 24399-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24399-1-AP (GPATCH4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GPATCH4 Polyclonal antibody

GPATCH4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24399-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

GPATC4, G patch domain-containing protein 4, G patch domain containing 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24399-1-AP targets GPATCH4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GPATCH4 fusion protein Ag20095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G patch domain containing 4
别名 GPATC4, G patch domain-containing protein 4, G patch domain containing 4
计算分子量 446 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC040147
基因名称 GPATCH4
Gene ID (NCBI) 54865
RRIDAB_2879523
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T3I0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPATCH4 (G patch domain-containing protein 4) is a 446 amino acid protein containing one G-patch domain. Existing as three alternatively spliced isoforms. This antibody is a rabbit polyclonal antibody raised against the 189 residues of N-terminal of human GPATCH4 protein

实验方案

Product Specific Protocols
WB protocol for GPATCH4 antibody 24399-1-APDownload protocol
IHC protocol for GPATCH4 antibody 24399-1-APDownload protocol
IF protocol for GPATCH4 antibody 24399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols