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GPATCH4 Polyclonal antibody

GPATCH4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24399-1-AP

Print datasheet

Synonyms

GPATC4, G patch domain-containing protein 4, G patch domain containing 4

验证数据展示

  • WB analysis of HEK-293 using 24399-1-AP

    WB analysis of HEK-293 using 24399-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24399-1-AP

    IHC staining of human liver using 24399-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 24399-1-AP

    IHC staining of human liver cancer using 24399-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50.

  • IF Staining of HEK-293 using 24399-1-AP

    IF Staining of HEK-293 using 24399-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24399-1-AP (GPATCH4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24399-1-AP targets GPATCH4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GPATCH4 fusion protein Ag20095 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G patch domain containing 4
Synonyms GPATC4, G patch domain-containing protein 4, G patch domain containing 4
Calculated Molecular Weight 446 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC040147
Gene Symbol GPATCH4
Gene ID (NCBI) 54865
RRIDAB_2879523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T3I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPATCH4 (G patch domain-containing protein 4) is a 446 amino acid protein containing one G-patch domain. Existing as three alternatively spliced isoforms. This antibody is a rabbit polyclonal antibody raised against the 189 residues of N-terminal of human GPATCH4 protein

实验方案

Product Specific Protocols
WB protocol for GPATCH4 antibody 24399-1-APDownload protocol
IHC protocol for GPATCH4 antibody 24399-1-APDownload protocol
IF protocol for GPATCH4 antibody 24399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols